Biography

Biography: Ronald Naumann

Abstract

Today, the mouse is a widely used animal model in the scientific community. The Transgenic Core Facility (TCF) at the MPI-CBG provides a centralized resource and stateof-the-art technology in production of Knock-Out (-IN) mice by injection or aggregation of embryonic stem cells into mouse embryos, and transgenic mice by injection of DNA or CRISPR into the cytoplasm or pronuclear of mouse zygotes. Our transgenic service facility generated about 70 mutant mouse lines per year. As backups in any cases, we use sperm freezing and embryo freezing to store all our mouse lines in safety conditions. We work very successful and efficient in full cost accounting. I would like talk about management, 3R's animal welfare, budget, animal and personal resources and the generating of mutant mice in a high health level (SPF). We show that using of fresh or frozen mouse embryos from breeding companies as donor for ES-cell injection is a flexible tool to provide customized projects. All the CRISPR, plasmid or BAC injections into zygotes will made in specific user background to reduce the numbers of re-crossing generations. An important role as facility leader is also the motivation of employees through it’s effective incentive plans so that the employees provide fullest co-operation.